Perbandingan Penetapan Kadar Kafein Pada Kopi Dengan Metode HPLC Dan Spektrofotometri UV-VIS
Abstract
Kopi merupakan minuman yang digemari semua kalangan dan merupakan minuman kedua yang paling banyak dikonsumsi di dunia. Kopi rasanya enak dan mempunyai bau khas, tapi tidak diperbolehkan minum kopi lebih dari 2-3 cangkir sehari, karena minum kopi terlalu banyak bisa menyebabkan masalah pencernaan. Karena mengandung kafein. Kafein merupakan turunan dari senyawa alkoloid. Oleh karena itu, terlalu banyak kafein tidak diperbolehkan karena dapat menyebabkan stimulasi, cemas dan susah tidur. Metode penelitian ini menggunakan metode penelitian Literatur Review Artikel (LRA) dalam bentuk jurnal nasional dan internasional 10 tahun terakhir.Tujuan dari penelitian ini adalah untuk mengetahui jumlah kafein pada minuman kopi dengan menggunakan metode UV-Vis dan HPLC, serta membandingkan sampel kopi yang dikumpulkan dengan metode spektrofotometri UV-Vis dengan sampel kopi dengan metode HPLC. menemukannya lebih tinggi kandungan kopi. Urutan UV-Vis efisien dan memenuhi persyaratan FDA, sedangkan metode HPLC lebih sensitif dibandingkan spektrofotometri UV-Vis. Hal ini tercermin dari nilai LOD dan LOQ yang rendah.
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